Pantothenate Assay Medium, AOAC M1281

Recommended by AOAC for microbiological assay of pantothenic acid or its salts using Lactobacillus plantarum ATCC 8014 as the test organism.

Composition**
Ingredients Gms / Litre
Casein acid hydrolysate 10.000
Dextrose 40.000
Sodium acetate 20.000
Dipotassium phosphate 1.000
Monopotassium phosphate 1.000
L-Cystine 0.400
L-Tryptophan 0.100
Magnesium sulphate 0.400
Sodium chloride 0.020
Ferrous sulphate 0.020
Manganese sulphate 0.020
Adenine sulphate 0.020
Guanine hydrochloride 0.020
Uracil 0.020
Riboflavin (Vitamin B2) 0.0004
Thiamine hydrochloride 0.0002
Biotin 0.0000008
p-Amino benzoic acid (PABA) 0.0002
Nicotinic acid 0.001
Pyridoxine hydrochloride 0.0008
Sorbitan monooleate complex 0.100
Final pH ( at 25°C) 6.7±0.2