Kligler Iron Agar is recommended for identification of Yersinia enterocolitica. It can also be used for the differential
identification of gram-negative enteric bacilli on the basis of the fermentation of glucose (dextrose), lactose and H2S
production.
Composition**
Ingredients Gms / Litre
Beef extract 3.000
Yeast extract 3.000
Peptic digest of animal tissue 15.000
Sodium chloride 5.000
Lactose 10.000
Dextrose 1.000
Ferrous sulphate 0.200
Sodium thiosulphate 0.300
Phenol red 0.024
Agar 15.000
Final pH ( at 25°C) 7.4±0.2

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